FastQCFastQC Report
Mon 27 Feb 2023
SRR3127398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025911
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251191.239886648525034No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG42580.21017705121300986TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC36640.18085690832420576TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG36020.17779655670954944TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24780.12231534356642516No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23010.11357853331168052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69700.086.781781
CGTAGGG4850.078.538572
GTAGGGT7850.075.4330753
ATAGGGC11400.074.204213
TAGGGCA10450.073.7544864
AGTAGGG31550.072.2906342
ATGCGGG9850.072.090892
TAGGGTA6350.070.308984
GTAGGGA11700.070.293173
GAATAGG11400.070.194371
AGGGATG22350.069.390075
TAGAGGG35500.068.3535462
TACAGGG12400.067.5053252
TACGACC4950.067.408416
AGTAAGG10350.067.309991
AGAGGGC30000.066.734323
ATAGCGG3400.066.45391
AGGGCAT15350.066.437345
CGAGGGA11500.065.79443
GGATAGG12950.065.791071
Produced by FastQC (version 0.11.5)