FastQCFastQC Report
Mon 27 Feb 2023
SRR3127397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976953
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337021.1320971476539938No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30140.10124446035930025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107250.084.046711
TAGGGCA16250.073.475894
ATAGGGA25000.072.7671663
ATAGGGC17350.072.06873
TAGAGGG52950.070.8437352
GAATAGG21050.070.811371
AGTAGGG50500.070.091952
ATAGCGG7550.069.1308441
CGTAGGG9900.067.8993152
TATAGGG25550.067.337152
AGGGATG39000.067.256485
GGTAAGG18700.066.88611
AAGAGGG112050.066.7036742
AGGGCAT27150.066.658485
TAGCGGG19700.066.573672
AATAGGG47250.066.556252
TAGGGAT18100.065.965934
TAGACGG4100.065.371261
AAGGGTA21700.065.203564
AGACGGG18500.065.047822
Produced by FastQC (version 0.11.5)