FastQCFastQC Report
Mon 27 Feb 2023
SRR3127396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557440
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286761.1212775275275275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94850.085.220761
ATAGCGG5950.076.089761
AGTAGGG42400.072.473812
GAATAGG14800.071.376991
TAGAGGG45800.070.798222
CGTAGGG7300.069.081262
ACGGGAT6550.069.076334
GTACGGG5200.068.8826142
ATAGGGC12750.068.3853
TAGCGGG15300.067.4608842
AGGGATG32700.067.400945
TAGGGCA12750.066.906414
AAGAGGG99600.066.7204742
AGACGGG16200.066.622332
AGTAAGG16500.066.595351
GGTAAGG15650.065.993581
AGGGCAT22950.065.934715
TAGGGTC8450.065.25724
GACCGAT1950.065.068398
AATAGGG38100.064.8194962
Produced by FastQC (version 0.11.5)