FastQCFastQC Report
Mon 27 Feb 2023
SRR3127394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625157
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258371.5898156301206592No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63870.393008183209376No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC30410.18712038283070498TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG29980.1844744846190245Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG29980.1844744846190245Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77000.086.337381
CGTAGGG5350.081.717652
CTACGAC10950.079.851955
TCCGCTA11600.077.0079961
AGGGATG28350.076.7740865
ATAGCGG2600.075.947961
AGGGTAC7500.073.96185
TACGACC11950.073.563176
CGCTACG11950.073.1697853
GTACGGG3100.072.7890852
TAGGGCA9900.072.651234
GCTACGA12100.072.262724
ACGACCA12100.072.262727
AGGGCAT13050.072.0453955
AGTAGGG24550.071.8069462
GAGGGTA10550.070.403034
GTAGGGC6750.070.340313
CGGGATG6350.070.329355
CGAGGGA9550.069.899123
TAGCGGG10450.069.727192
Produced by FastQC (version 0.11.5)