FastQCFastQC Report
Mon 27 Feb 2023
SRR3127393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427906
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205801.4412713441921248No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55560.3891012433591567No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG41200.2885343993232048Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG36300.254218414937678Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC35600.24931613145403128TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64100.087.007261
CTACGAC8350.081.0435945
CGTAGGG4350.080.1712652
GCTACGA8600.078.36354
TAGGGTA5450.076.960834
TCCGCTA8700.076.94811
CGCTACG8800.076.5825043
TAGGGCA7950.076.471374
AGGGATG23700.075.943945
TACGACC9100.075.397016
GTAGGGT5600.074.057813
GATGCGG2300.073.791011
AGGGTAC6650.073.494425
ATAGCGG2250.073.335511
GTACGGG1550.072.9719242
AAGGGCA19650.072.430364
AGTAGGG19650.071.710852
TAGCGGG6800.071.3846052
AGGGCAT10900.071.137755
CGAAGGG13250.071.136152
Produced by FastQC (version 0.11.5)