FastQCFastQC Report
Mon 27 Feb 2023
SRR3127391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014120
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258711.2844815601850932No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72470.3598097432129168No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG23570.11702381188807022No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22000.10922884435882668No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG21900.10873234961174111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79050.085.35081
ACGGGTA2550.081.115784
AGGGATG45550.078.642885
ATAGCGG3850.078.158611
GGATGGC39150.074.453517
CGAGGGA12050.073.343843
AAGGGAT41600.072.210514
CTACGAC13900.072.037345
AACGAGG7800.071.1287161
TCCGCTA14300.070.690321
GAGGGAT37450.070.54684
ACGACCA13750.070.4351967
CGGGATG8250.069.5182345
TAAGGGA20150.069.290593
TAGGGCA13400.069.112134
AGGGCAT18700.068.881375
AGTAAGG12800.068.6893161
TATAGGG17800.068.66672
TAGCGGG9750.068.4662552
AGGGTAT11700.068.305545
Produced by FastQC (version 0.11.5)