FastQCFastQC Report
Mon 27 Feb 2023
SRR3127386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065572
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408621.332932320624014No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32220.10510273449783597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121950.085.887741
ATAGGGC17500.074.120443
TAGGGCA17200.072.13444
AGGGCAT25800.070.312835
ATAGCGG7500.068.414511
CGTAGGG9300.068.2766342
GAATAGG18500.068.193981
AGGGATG45600.067.815355
ATAGGGA28700.067.629333
GTACGGG8700.067.579042
ATAGAGG21550.067.280071
AGGGTAC14900.066.867735
AAGAGGG112000.066.856912
GCATAGG8500.066.4576951
ATAACGG2700.066.252591
AGTAGGG49400.066.172742
GTAGGGT10150.065.7490163
GATAGGG44400.065.0439152
TAGGGTA11850.065.041794
CGAAGGG32250.064.755182
Produced by FastQC (version 0.11.5)