FastQCFastQC Report
Mon 27 Feb 2023
SRR3127381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610377
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194601.2084126884574233No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG22100.1372349456059047No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG20950.13009376065356124No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20900.12978327435128545No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19000.11798479486480495No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC18360.11401057019567468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59250.086.5310061
CGTAGGG4900.075.0410842
AGGGCAT14250.072.221375
GAATAGG10100.071.916721
ATAGGGC10550.071.4937443
ACGGGTA1650.071.426044
TAGGGTA7300.071.034674
TAGGGCA7500.071.0260544
AAGGGTA11400.069.884744
AGGGTAC8950.069.83375
AGTAGGG28650.069.4365842
GGATAGG9050.069.316021
AGTACGG2050.069.023651
TAGAAGG13250.068.346441
ATAGAGG9900.068.128911
GATAGGG21900.067.160052
ATGAGGG25400.066.9998862
TAGAGGG30250.066.854782
ATAGCGG4050.066.3821
TAAGGGA13600.065.859013
Produced by FastQC (version 0.11.5)