FastQCFastQC Report
Mon 27 Feb 2023
SRR3127378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075893
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333391.0838803560461954No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61420.1996818484908285No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC37970.12344382590681795TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG35730.1161613879286438No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG32480.10559535068352507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107100.084.954261
ATAGCGG6700.080.959061
TAGGGCG5250.074.5220644
CGTAGGG8300.073.2618262
TACGGGT3100.072.987023
TAGGGCA14500.072.819244
GAATAGG21050.071.4794161
ACGGGTA3300.071.420394
TAGCGGG16600.071.27412
CTACGAC11900.071.082465
AGTAGGG46950.070.781492
TAGAGGG58000.070.218552
ATAGGGA27300.069.929193
AGGGTAC15600.069.285145
TAGGGTA12300.068.981644
ACGGGTC3150.068.835654
ATAGGGC23500.068.800633
AGGGATG41250.068.695815
AGGGCAT30900.068.436995
CGAAGGG28900.068.3411562
Produced by FastQC (version 0.11.5)