FastQCFastQC Report
Mon 27 Feb 2023
SRR3127371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886522
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416181.441804358324655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT133700.086.276251
GAATAGG17000.071.148151
TAGAGGG53450.070.907172
AGGGATG41950.070.8035055
ATAGGGA25500.070.403273
ATAGGGC18450.069.794923
AGTAGGG46800.068.925692
ATGTAGG11250.068.1890261
GTAGGGA22100.068.049873
GAGGGAT34550.068.012944
ACGGGTA3950.067.8183754
AGAGGGC42800.067.750273
TAGGGCA16050.067.6404654
TATAGGG22550.066.936252
AGGGAAT30650.066.547065
AAGAGGG106350.066.4102862
AGGGCAT24400.066.257995
ATAGAGG19700.066.174841
AATAGGG43950.065.6919862
CGGGTAT3450.065.387055
Produced by FastQC (version 0.11.5)