FastQCFastQC Report
Mon 27 Feb 2023
SRR3127358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225565
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230721.036680573247692No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC66880.3005079608998164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85450.083.4109341
AGGGATG51050.075.8733445
CGTAGGG6900.074.9378742
ATAGCGG4750.073.244321
GTACGGG4750.072.241552
AGAGGGC35550.071.13783
AGTAGGG35100.070.4427642
TAGAGGG44300.070.3505552
ATAACGG2350.070.022251
ATGCGGG11750.069.60962
TAGGGTA8750.068.763764
TACGACC11400.068.448046
TAAGGGA24850.068.287093
AAGAGGG84350.068.0994952
AGGGCAT23400.068.099145
GGATGGC44250.068.0945367
ATAGGGC15100.067.863663
TAGGGAT19400.067.8444064
ACGGGTA3000.067.375964
AATGCGG4750.067.3055951
Produced by FastQC (version 0.11.5)