FastQCFastQC Report
Mon 27 Feb 2023
SRR3127356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956132
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183450.937820147106637No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56300.28781288788282183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67500.084.394931
AGGGATG44750.077.190255
ATAGCGG3250.076.776311
CGTAGGG5550.072.8946462
TACGACC10800.072.6709446
AGAGGGC30450.072.540193
CTACGAC10950.071.675455
AGTAGGG31200.071.317592
GGATGGC38850.070.5254067
ATGCGGG11000.069.708392
AAGAGGG71650.069.595282
TAGAGGG37600.069.3125842
ATAGGGC11900.068.717983
AGGGAAT25100.068.3419345
GTACGGG4900.068.163582
AGGGCAT20550.068.151055
TAGGGCA12350.068.116794
ACGACCA11550.067.952057
ATAGGGA21300.067.737143
ACGGGAT9600.067.557874
Produced by FastQC (version 0.11.5)