FastQCFastQC Report
Mon 27 Feb 2023
SRR3127350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409488
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160041.1354477654297164No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG23760.16857185020376192TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG22370.15871011317584824TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC21430.15204102482603613No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19430.13785147514558477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49050.086.2551961
GTACGGG2600.077.763212
CGTAGGG4700.077.032132
ACGGGAT3900.074.7124
TAGGGCA7000.073.1798864
TAGGGTA3350.072.949414
AGTAGGG24500.071.2011262
CGAAGGG16500.070.101972
GTAGGGA9850.069.182313
ATAGGGA14700.069.0557253
GAGGGAT15000.068.927844
GGTAAGG7700.067.841711
GAATAGG10150.067.6941761
AGGGCAT12500.067.6746145
TAGAGGG23500.067.0279542
ACGGGTA1200.066.5780264
TATAGGG11800.066.544712
ATGAGGG22500.066.4543842
TGTAGGG9700.066.4091342
AGGGATG19500.066.276775
Produced by FastQC (version 0.11.5)