FastQCFastQC Report
Mon 27 Feb 2023
SRR3127343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021051
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204871.0136805058358251No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC65570.32443515774713255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70800.082.609381
AGGGATG55850.079.113435
CGTAGGG6550.078.222332
TAAGGGA22550.076.500643
ATAGCGG5200.075.9483261
GGATGGC47000.075.210117
TAGCGGG11800.072.499492
CGAGGGA15600.070.507743
TAGGGTA7200.070.507744
ATGCGGG10200.070.507742
GAGGGAT40750.070.4789054
AAGGGAT56500.069.6341864
ATGGCAC47250.069.4262859
TAGAGGG37350.069.343622
GGGATGG57350.068.929976
AGGGCAT21900.068.683345
AAGGGTA16850.068.624744
GTACGGG5350.068.530882
TAGGGCA12550.068.166844
CGGGATG11200.067.989615
Produced by FastQC (version 0.11.5)