FastQCFastQC Report
Mon 27 Feb 2023
SRR3127336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367918
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241401.0194609779561623No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG26750.11296843894087549Illumina PCR Primer Index 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26120.1103078738368474No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC25970.10967440595493594TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG24240.10236840971689053RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79400.083.789891
AGGGATG36600.074.214665
ATAGGGA19850.072.173433
AGTAGGG40550.071.474082
AGAGGGC33750.071.352873
TAGAGGG41550.071.114942
CGTAGGG7950.070.541422
ATAGAGG15200.070.105151
ATAGCGG5250.070.051971
TAGGGAT16650.069.6282044
AGGGCAT21050.069.20745
GTAGGGA20850.069.163933
TAGGGCA14450.069.140474
ACGGGAT6250.068.616044
AGGGTAT15050.068.071135
GAATAGG14100.067.883151
GGTAAGG14450.067.870421
ATAGGGT9500.067.4651643
TAGGGAA15550.067.280144
TAGCGGG11900.066.9274142
Produced by FastQC (version 0.11.5)