FastQCFastQC Report
Mon 27 Feb 2023
SRR3127335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425617
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231170.9530358667506041No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC27520.11345566921735789TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG27520.11345566921735789Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG26420.10892074057858268RNA PCR Primer, Index 10 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24890.10261306710828626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76550.083.586651
CGTAGGG7650.074.404822
TAGGGCA13750.072.803294
AGGGATG38200.072.2185755
ATGCGGG11300.070.769752
AGTAGGG44950.070.744742
AGAGGGC35350.070.595863
AATGCGG5850.070.020641
ATAGGGC14950.069.788833
TAGAGGG44350.069.47442
TAAGGGA23600.069.102163
AGGGCAT20550.068.838095
GTAGGGA21250.067.676313
AGGGAAT27650.067.649115
AAGGGTA17700.067.1771244
GTAAAGG19900.066.7204061
GAATAGG15400.066.6497341
AGGGTAT14400.066.253335
ATAGGGT10400.065.9771653
AAGAGGG86850.065.9170762
Produced by FastQC (version 0.11.5)