FastQCFastQC Report
Mon 27 Feb 2023
SRR3127322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049627
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459411.5064465260833537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153500.086.6673361
AGTAGGG54900.072.431822
TAGAGGG56250.070.275652
ATAGGGC17450.069.495343
TAGGGTA9950.069.442554
AGATAGG20250.068.505881
TAGGGCA17350.067.186754
AGGGATG39700.067.1311345
ATAAGGG44950.066.191992
ATAGGGA24250.066.0958253
AGAGGGC49400.065.843153
ATAGCGG7800.065.714741
TAGGGTC9600.065.609244
GAATAGG18300.065.269991
ATGCGGG16250.065.0819552
TAGTAGG16350.065.001231
ATAGAGG20350.064.934091
AAGAGGG119550.064.912592
GATAGGG47000.064.9050142
AGTAAGG20000.064.659681
Produced by FastQC (version 0.11.5)