FastQCFastQC Report
Mon 27 Feb 2023
SRR3127313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968837
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355241.196562829148249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118750.086.200881
ATAGGGC18350.073.2593843
GAATAGG18150.071.761261
GTAGGGT8850.071.169653
TATAGGG25750.070.095032
CGTAGGG6450.069.959192
ATAGCGG5150.069.389431
AGATAGG17450.068.9813161
AGGGATG43650.068.810895
AGGGCAT23650.068.370365
TAGGGCA16250.068.2651754
GGTAAGG16650.067.7772451
TAGAGGG56700.067.728622
TAAGGGA24800.067.662773
AGTAGGG49250.066.616592
AGTAAGG19400.066.410351
ATAGGGA29150.065.9504853
TAGGGTA9200.065.908664
AAGAGGG114900.065.903542
AGAAGGG112000.065.427662
Produced by FastQC (version 0.11.5)