FastQCFastQC Report
Mon 27 Feb 2023
SRR3127309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123064
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238381.122811182328936No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57900.27271905133335594No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC32840.15468210096351312No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG30100.14177622530455983No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG26210.12345365000772468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76600.084.461911
AGGGATG37200.075.417315
AGGGCAT19200.074.896225
GTAGGGT8350.074.508793
TAGGGTA9050.073.953684
ACGGGTA2000.073.05534
AAGGGTA15450.072.300264
TAGGGCA12750.070.606264
GAGGGAT30400.070.233554
CGTAGGG4850.069.9698262
TAAGGGA19950.069.458353
ATAGCGG4350.069.378711
AGTAGGG37100.069.364742
TAGGGCG2800.069.015374
TAGACGG4100.069.008551
CGAAGGG20050.068.8768542
AGGGTAC9200.067.936555
CTACGAC12050.067.858125
TCCGCTA12200.067.641581
AGACGGG17400.066.906412
Produced by FastQC (version 0.11.5)