FastQCFastQC Report
Mon 27 Feb 2023
SRR3127305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848781
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214321.1592503384662651No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG39800.21527698521350017No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC39350.21284294894852337No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG34210.18504084583301103No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTGTACAG19120.1034194964141237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66050.085.2475051
TAGGGCA8750.077.88094
CGTAGGG4600.072.5900342
TAGAGGG31900.071.650972
AGTAGGG28300.071.459262
AGAGGGC23750.071.23773
AGGGCAT15900.070.939015
TAGGGTA7600.070.4956364
AGGGTAA8350.068.103575
TAAGAGG12450.068.0654141
TGTAGGG12300.068.059912
GGTAAGG11850.067.936181
AGGGAAT20650.067.8214655
ATAAGGG24050.067.269752
TATAGGG14550.067.2321242
ATAGGGC9750.066.518963
TAGCGGG9000.066.3647842
AGGGATG23700.066.2321855
ATAGAGG13650.066.220421
AAGGGTA13850.066.16924
Produced by FastQC (version 0.11.5)