FastQCFastQC Report
Mon 27 Feb 2023
SRR3127298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688894
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT621432.3110989127871906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT206750.088.321391
ATAGGGC15250.071.2035453
CGTAGGG8200.070.512
AGTAGGG46850.069.933082
TATAGGG22600.069.054052
AGAGGGC38700.067.655593
TAGAGGG49400.066.989262
ATAGGGA25200.066.965843
GATAGGG40750.065.7516562
GAATAGG17000.065.54381
AGTAAGG19300.065.528041
AAGAGGG95450.065.252842
AAGGGGC84050.065.154984
TAGCGGG17350.065.0236362
AGGGTAC10650.064.882455
ATAGAGG18800.064.519951
GTAGGGA20550.064.50553
TAGGGCA14300.064.428724
TAGTAGG14100.064.353231
ATAAGGG35400.064.2690052
Produced by FastQC (version 0.11.5)