FastQCFastQC Report
Mon 27 Feb 2023
SRR3127296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352821
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514312.1859291463311488No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT26600.11305577432367357No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT26410.1122482330785045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT171200.088.334411
TAGGGCA11200.073.431344
CGTAGGG6650.072.823392
AGTAGGG37650.071.555812
AGGGATG35200.070.62765
AGTAAGG14900.069.474311
ATAGGGC13550.069.020043
GAATAGG14000.068.563031
TAAGAGG16500.068.440811
TAGAGGG44750.067.557442
ATAGAGG18350.066.156341
ATAGGGA19450.065.9636153
GTAGGGA16250.065.0714653
AGGGCAT18600.064.935425
GTAGGGC12400.064.8090743
GTAAAGG17150.064.74941
TAGTAGG10500.064.529911
GTACGGG5350.064.154092
AAGAGGG84150.064.142082
GTTTTTT238700.064.094452
Produced by FastQC (version 0.11.5)