FastQCFastQC Report
Mon 27 Feb 2023
SRR3127294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269632
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283541.24927741589826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97650.086.44641
TAGGGCA11300.071.31434
ATAGGGA22050.070.7421653
ATGTAGG8400.070.738751
ATAGGGC13400.069.2823
GAATAGG16150.068.62171
AGGGATG32200.068.447755
TAGGGAT15100.067.411784
ACGGGTA2450.067.322614
ATAGCGG4850.067.092421
TAGAGGG43800.067.030572
AGTAGGG37900.066.771952
TAGGGTA6850.066.7329254
GTAGGGT8950.066.3447343
GATAGGG33750.066.0459752
AGATAGG13500.065.673511
CGTAGGG7500.065.347812
GAGGGAT25700.065.0959854
ATGCGGG12700.064.9371642
ATAAGGG29250.064.928922
Produced by FastQC (version 0.11.5)