FastQCFastQC Report
Mon 27 Feb 2023
SRR3127291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777705
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189491.0659248863000328No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27320.15368129132786373No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG25130.14136203700839003Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG22240.12510512149091102Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC21720.12218000174382139Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69800.085.258391
TAGGGCA8700.078.00664
ATAGGGC11700.077.339893
CGTAGGG4400.074.977942
ATAGGGA14400.071.675343
TAAGGGA15200.069.7633063
CTACGAC5600.069.65155
ATAGAGG12600.069.236391
ATAACGG2050.069.008191
AGGGATG21250.068.776795
AGGGCAT14000.068.4766545
ACGGGAT3400.067.9211964
AGATAGG10300.067.7575451
AGTAGGG25450.067.5916752
AAGGGAC17200.067.131424
GATAGGG25300.067.061012
ATAAGGG24250.067.049492
AGAGGGC28600.066.738613
CGAAGGG17800.066.721942
AGGGAAT18550.066.6273045
Produced by FastQC (version 0.11.5)