FastQCFastQC Report
Mon 27 Feb 2023
SRR3127288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977837
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232061.1733019455091598No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG26140.1321645818133648No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC24700.12488390094835923TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG23340.11800770235363177No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22560.11406400021842042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71050.085.7689361
TAGGGCA10000.073.068794
GAATAGG12050.070.847271
TAGAGGG32500.070.6392062
AGTAGGG30800.070.558682
TACGGGT1700.069.3252263
AGGGAAT23750.068.8576665
CGTAGGG6450.068.7018362
AAGGGTA14050.067.440384
TAAGGGA16100.067.344513
AGGGCAT16400.067.3380665
GGTAAGG12400.067.326051
ATGCGGG9850.067.0026552
ATAGGGA15200.066.6799243
AATAGGG28900.066.5522162
ATAGGGC12550.066.485933
TATAGGG15150.066.2776642
AGGGATG27350.065.979745
CGAAGGG19750.065.878272
GTACGGG4950.065.7119142
Produced by FastQC (version 0.11.5)