FastQCFastQC Report
Mon 27 Feb 2023
SRR3127275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378532
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220760.9281355054293993No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25030.1052329756337102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78050.081.7901461
ATAGCGG5450.074.2875751
CGTAGGG7500.072.752052
ATAGGGC13450.072.678513
TAGGGCA11100.071.976534
AGTAGGG37950.071.889392
GAATAGG14650.070.375371
TAGAGGG44700.069.767722
ATAGGGA19850.067.712753
GTAGGGT9650.067.2073443
AGTAAGG16600.066.078781
GGATAGG13400.066.049161
AAGAGGG83950.065.556192
TAGTAGG8550.065.523191
AAGGGTA17000.065.518564
ATAGAGG16200.065.3855441
AATAGGG33900.065.2148742
AGGGTAC10400.064.62015
GTAGGGC12700.064.388823
TACGACC5500.064.0860446
Produced by FastQC (version 0.11.5)