FastQCFastQC Report
Mon 27 Feb 2023
SRR3127273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931955
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225141.1653480541731045No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG23970.12407121283880836No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG21360.11056158140329356No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC20370.10543723844499485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67550.086.1840061
CGTAGGG5750.077.032862
ATAGGGC10200.072.529633
ACGGGAT4750.071.4257664
TAGAGGG32400.071.263492
CGAAGGG18650.070.9975742
ATAAGGG29350.069.196662
AGTAGGG31000.068.85772
TAGGGTC4950.068.539874
AGATAGG12550.068.361681
GAATAGG13700.068.128641
AGAGGGC28900.067.176193
TAGGGCA8800.066.933474
TAAGGGA15000.066.912053
AGGGATG22250.065.8976755
AAGGGGC53850.065.890874
AGTAAGG11400.065.7472151
GATAGGG29100.065.257142
AAGAGGG66400.065.146432
AGGGCAT17050.065.0478745
Produced by FastQC (version 0.11.5)