FastQCFastQC Report
Mon 27 Feb 2023
SRR3127271.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127271.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014719
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144961.4285728364207233No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC73060.7200022863472547No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG21130.20823498919405273RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC20440.2014350771001627RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG19010.1873425056592022Illumina PCR Primer Index 11 (95% over 24bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16510.16270514300018035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1100.089.746844
CGTTTTT59550.088.1744161
AGGGATG31950.084.6037455
GTACGGG1800.083.5737842
GGATGGC28150.083.165567
GATGGCA26950.082.507798
GTTACGG800.082.263881
CGCTACG12150.080.4784553
CGGGATG6550.080.383945
TAAGGGA12350.079.936463
CGTAGGG2600.079.555812
TAAGCGG1300.079.5518951
CGGGTAC950.079.175165
AGGGAAT19100.079.006765
TACGGGT600.078.350423
GCGGGAT6300.078.350424
TCCGCTA12300.078.346561
GCTACGA12500.078.225064
GAATGCC13750.077.9515468
AAGGGAT30300.077.729824
Produced by FastQC (version 0.11.5)