FastQCFastQC Report
Mon 27 Feb 2023
SRR3127267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759859
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85961.1312625105447194No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55130.7255293416278547No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC16510.2172771527349153No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG16320.2147766888330598No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG16070.21148660475167103No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCCT9350.12304914464394053No Hit
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA8540.11238927222024087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG800.088.3979341
CGTTTTT36850.088.150051
AGGGCAT7700.082.39135
AGGGATG20350.081.517075
TAGGGCA5000.081.052914
CGCTACG9550.080.9246143
TCCGCTA9650.080.611581
ACGGGTA1300.079.747954
GCTACGA9700.079.67324
CGTAGGG1900.079.366382
GGTAAGG4400.079.290271
GACCGAT950.079.147038
CGGGAAT1900.079.147035
AACGCGG1550.079.0828861
GGATGGC14600.078.859047
AGGGATT12700.078.8159565
ATATCGG301.4291418E-678.5759351
CTACGAC9800.078.162745
ACGACCA9800.077.683227
TAGCGGG3350.077.367412
Produced by FastQC (version 0.11.5)