FastQCFastQC Report
Mon 27 Feb 2023
SRR3127262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691952
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303671.1280661765142914No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52890.196474528520568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98650.084.4978941
ATAGAGG19750.072.128981
AGGGATG48400.070.509045
TAGAGGG56650.070.446812
CTACGAC10000.070.038985
TAGGGTC8000.069.333894
GAATAGG18900.069.153991
TAGGGCA12850.069.137274
AGTAGGG50900.067.415322
TACGACC10400.067.345186
AGGGCAT23450.066.7505655
TGTAGGG19400.066.6322562
ATAGCGG7100.065.555791
AGAGGGC40900.065.509623
CGTAGGG9550.065.463892
AGGGTAC13550.065.21875
AGAAGGG101250.065.042422
AAGAGGG95100.064.997822
ATAGGGA24950.064.621513
ATAGGGC14200.064.550543
Produced by FastQC (version 0.11.5)