FastQCFastQC Report
Mon 27 Feb 2023
SRR3127259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236405
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261941.1712547593123785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80500.084.696931
CGTAGGG7950.073.316952
ATAGGGC13000.071.954763
TAGGGCA13250.069.5328454
TAGTAGG9000.069.4871751
GAATAGG13200.069.107211
AGGGTAT13950.069.076375
GGTAAGG12600.068.292981
AGTAGGG37800.068.145722
TAGAGGG43600.068.136592
TAGCGGG13750.067.68812
GGATAGG14000.067.5092851
AGGGCAT20650.067.1508945
ATAGCGG4850.066.8964161
AGTAAGG17100.066.819881
TAACGGG8600.066.6823962
AAGAGGG85050.065.934992
AACGAGG11300.064.91451
TGTAGGG17700.064.798712
AAGGGCA34450.064.5389254
Produced by FastQC (version 0.11.5)