FastQCFastQC Report
Mon 27 Feb 2023
SRR3127257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960473
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211651.0795864059336702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68300.085.595511
ATAGGGC9400.072.995983
CGTAGGG6550.072.5229342
TAGGGCA10200.070.956884
TAGGGTA6050.069.91354
AGTAAGG14400.069.625331
AATAGGG26700.069.2271652
GTAGGGT7000.069.1533363
ATAGGGA13600.068.768273
GGTAAGG11500.068.764071
AGTAGGG30800.068.715872
TAGAGGG36200.068.33962
GTATAGG6850.068.02911
TAGACGG4250.067.560221
GAATAGG11650.067.474661
AAGAGGG73500.067.444812
ATAGCGG5700.066.889861
TAGCGGG12950.066.4624942
TAGTAGG7650.066.452671
TAAGAGG14900.066.341181
Produced by FastQC (version 0.11.5)