FastQCFastQC Report
Mon 27 Feb 2023
SRR3127246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2838393
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324721.1440276240816547No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC32560.11471279699463748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109150.086.0846561
TAGGGCA14200.074.364644
CGTAGGG8000.072.480622
AGGGATG42550.071.566645
AGGGCAT25150.070.629975
ATAGGGC16350.069.7757343
TAAGGGA24050.068.997663
TATAGGG23300.068.790712
AGTAGGG47150.068.788132
GAATAGG16950.068.7364961
ATAGGGA25850.066.928693
ATAAGGG40550.066.7310262
AGTAAGG17100.066.478481
CGAAGGG34200.066.3018652
AAGGGCA41000.066.1135944
ATGAGGG47000.066.0989152
TAGAGGG49150.066.084942
ATAGCGG6150.065.960431
ATAGAGG19950.065.493321
AGGGAAT28800.065.4315265
Produced by FastQC (version 0.11.5)