FastQCFastQC Report
Mon 27 Feb 2023
SRR3127245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894359
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315791.091053321305339No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC34370.118748227154959No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28980.1001257964198636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106900.085.334611
AGGGATG40950.072.873675
TAGGGCA15300.071.874574
AGTAAGG16650.070.393771
TAAGGGA25100.069.837063
ATAGGGC17900.069.048393
AGTAGGG48100.067.363592
TAGAGGG48950.067.250632
CGTAGGG7500.067.092442
GAATAGG18950.067.066191
TATAGGG22050.066.755462
ATAAGGG39550.066.4685752
GTACGGG6100.066.3009342
CGAAGGG31300.066.108822
ATAGGGA24250.066.083573
ACGGGTA3350.065.933164
TAGGGTA9650.065.744174
AGGGTAC12100.065.637525
AATAGGG43450.065.372962
AGGGCAT26000.065.250635
Produced by FastQC (version 0.11.5)