FastQCFastQC Report
Mon 27 Feb 2023
SRR3127243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061268
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252271.2238583240995349No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44990.21826370952248808No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC29110.14122375159367923No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG26930.1306477372180619No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG24880.12070240259878871No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22340.10837989043637217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76100.084.76591
AGGGATG49100.078.291435
AGTAGGG31800.073.653852
ACGGGTA2700.073.308054
GGATGGC39750.072.4712457
AGATAGG10950.071.48481
ATAGGGA20350.071.095173
TAGGGCG3400.070.68994
TAGGGCA11300.070.4813844
ATAGGGC13900.069.842313
GAGGGAT36650.069.821954
AAGGGAT39250.069.519254
TAGGGAT19200.069.462654
GGTAAGG13300.069.135761
TATAGGG16850.069.0817262
ATAGCGG4100.069.006051
TGTAGGG16000.068.333572
GTAGGGC9950.068.203323
CGGGATG9800.068.0933465
TAGCGGG10050.067.99362
Produced by FastQC (version 0.11.5)