FastQCFastQC Report
Mon 27 Feb 2023
SRR3127241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769842
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380981.3754575170713708No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34310.12386988138673614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127950.086.140521
ATAGGGC16750.076.049373
TAGGGCA14900.073.18854
GGTAAGG16200.071.4019851
AGGGATG41950.070.815165
TAGAGGG49000.070.2192462
ATGTAGG6950.069.685531
AGTAGGG49650.069.0159452
TAAGGGA21650.068.173083
AGAGGGC40900.067.4614943
ATGCGGG13600.067.396422
GTAGGGA18650.065.529323
AAGGGTA18450.064.965834
AAGAGGG103900.064.874612
AGGGCAC23900.064.705195
CGTAGGG8650.064.6654052
ATAAGGG37050.064.4490662
ATAGGGA23100.064.0973053
AGGGCAT25750.063.8898625
GAGGGAT35400.063.867954
Produced by FastQC (version 0.11.5)