FastQCFastQC Report
Mon 27 Feb 2023
SRR3127236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761436
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314491.1388639823627997No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52580.19040817893298995No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC32140.11638871949232212No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG31280.11327439781331163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114400.085.076671
AGGGATG42500.074.31095
CGTAGGG6600.074.1072162
GTACGGG6700.072.29922
TAGGGCA15200.071.114214
TAGAGGG48550.070.520162
CGAAGGG25250.069.845912
GAGGGAT37700.069.560874
ACGGGTA2250.068.929254
TAAGGGA24400.068.762333
TAAGAGG20000.068.712991
AGTAAGG17600.068.4562761
ATAGGGC14650.068.330433
ATAGGGA23200.068.267523
GGTAAGG17700.068.069521
CTACGAC12300.067.2480555
ATAGAGG20250.067.167441
AGAGGGA62950.067.04293
AAGGGTA17000.066.9019244
AAGAGGG101400.066.8803252
Produced by FastQC (version 0.11.5)