FastQCFastQC Report
Mon 27 Feb 2023
SRR3127233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527719
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60581.147959425376005No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38790.7350502824419815No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC31840.6033514048196105No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG28350.5372177238265062No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG26220.4968553339940385No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG24530.46483071483118854No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22080.41840449178445344No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10790.2044648761935803No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT10190.19309518891682884No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT8800.16675541339235464No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATG7780.14742694502187717No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT7680.1455319971424186No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG7640.14477401799063516No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATT7240.13719422647280088No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT7060.1337833202897754No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCATTAGACGTC7060.1337833202897754No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6830.1294249401670207No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCC6610.12525605483221183No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACGTCG6560.12430858089248255No Hit
TAGACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT6190.11729727373848582No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6180.11710777895053996No Hit
AAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT5710.10820152391708465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGGG201.833315E-594.037732
AATCGGG301.3207682E-894.037732
GGTCGAC254.9196024E-793.993187
TACGGGT353.583409E-1093.993173
CGTTTTT33900.088.418141
AGTACGG600.086.266681
AGGGTAC2150.085.249625
CGCTACG6950.084.526243
AGGGATG12150.083.93635
GCTACGA7150.082.819174
GTACGGG750.081.499372
CGTAGGG700.080.603772
GGATGGC9100.080.565587
CGGGTAT353.862442E-880.5655755
CTACGAC7400.080.021225
GATAGGT650.079.532698
TCCGCTA7700.078.831641
AGGGCAT4300.078.6919565
AGGGAAT11000.078.612475
ACGACCA7500.078.327657
Produced by FastQC (version 0.11.5)