FastQCFastQC Report
Mon 27 Feb 2023
SRR3127227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7869974
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750843.49536097577959No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT307340.3905222558549749No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT255090.3241306769247268No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT251750.31988669848210427No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT243260.30909886106358164No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC216640.27527409874543424No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG196540.2497339889559991TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC131980.1677006810950074No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT124020.15758628935749977No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC123570.15701449585475122No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC122910.15617586538405337No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT107890.13709066891453517No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC84010.10674749370201222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT819050.090.721991
AGGGACT169150.070.433975
ATAGGGC35250.070.3969353
GACTTAA133850.070.1163568
GGTAAGG44000.070.111551
GGATAGG39600.068.8765261
ACTTAAT135750.068.6503149
TAGGGAC33050.068.541654
AGATAGG38200.068.0769651
AGTAGGG111700.067.868532
GGACTTA139650.067.745287
ATAGGGA44050.067.216143
TAGTAGG24850.066.8015751
GTAGGGA50450.066.328133
AAGGGGC261200.066.214584
ATAGAGG49450.066.188321
GTTTTTT1125100.066.051542
AATAGGG91750.065.772772
GATAGGG102900.064.67482
AGTAAGG44950.064.654271
Produced by FastQC (version 0.11.5)