FastQCFastQC Report
Mon 27 Feb 2023
SRR3127217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605534
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257710.9890870738973278No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG57550.22087602771639134No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC47910.18387785382957966No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG43490.16691396082338592No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35710.1370544387446105No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30610.1174807160451562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79350.084.244941
TAGGGCA13550.076.1409764
AGGGCAT25450.073.676785
AGGGATG39150.072.382085
GAATAGG14750.071.5929951
ATAGGGC16850.071.573733
CGTAGGG8050.071.396612
ATAGGGA21950.070.182183
AGGGAAT32050.069.501775
TAGGGCG3400.069.2794954
TAGAGGG42850.067.944032
AGTAGGG37650.067.943592
GATAGGT4500.067.880718
AAGGGTA17700.067.604264
GTAGGGA21700.067.517183
TAAGGGA20600.067.463433
GTAGGGT11550.067.300093
ACGGGTA2100.067.300084
ACGGGAT5900.067.0719454
GAGGGAT34050.066.825724
Produced by FastQC (version 0.11.5)