FastQCFastQC Report
Mon 27 Feb 2023
SRR3127208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479053
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49981.04330836045281No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG25600.5343876356060812No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG23790.49660475980736996No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC22240.464249258432783No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8410.175554688103404No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7620.1590638196608726No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT6880.14361667706913434No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG5660.11814976630978201No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5610.11710604045898887No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5570.11627105977835438No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5450.11376611773645089No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT5370.11209615637518186No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5210.10875623365264386No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5180.10812999814216798No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5050.10541631093010585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG156.817322E-494.284581
GTACGGA156.834363E-494.225382
CGTTTTT21100.088.252151
GGGCGAT1150.085.816376
TAGGGCA2300.083.98354
AGTACGG452.7284841E-1183.808511
ATAGCGG700.080.8153461
ATAGGGC3450.079.203953
TACGAGG900.078.570481
GAATAGG2350.078.236141
ACGGGTA850.077.597384
AGGGAAT11000.076.473165
ACGGGAT1300.076.105124
AGGGTAG1050.076.086625
GTGTAGG950.074.4351961
ATGACGG700.074.0807341
GGGAATG13800.073.5568856
CGTAGGG1350.073.2864152
ACGGGTC452.579327E-973.286414
GTAAAGG3300.072.856261
Produced by FastQC (version 0.11.5)