FastQCFastQC Report
Mon 27 Feb 2023
SRR3127207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507673
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53011.0441760739688737No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG25170.49579158237684495No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG22880.4506838063083914No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC22560.4443805362900922No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8630.16999131330600603No Hit
AGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7930.15620291014097656No Hit
GGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT7300.14379334729245005No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG6050.11917119878346888No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT5750.1132618831413134No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5530.10892838500373271No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5430.10695861312301422No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5410.10656465874687052No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5400.10636768155879868No Hit
GGAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT5090.10026138872857135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG254.88315E-794.1097641
CGGGAAT1500.087.723325
CGGGTAT700.087.275745
CGTTTTT22250.085.0160141
AGGGCGT850.082.93175
TACGGGT401.035005E-982.24063
AGGGTAT3450.081.72985
CGAAGCT353.850073E-880.601922
GACCGAT353.8633516E-880.5622258
ACGGGAT1400.080.5622254
GGGAATG12050.080.339376
ATAGGGC2300.079.6865543
GTACGGG1300.079.5685652
AAGGGAC3450.079.005474
CGAAGGG4350.077.822552
CGGGGGA4900.077.6850055
AAGGGTA2850.077.499924
GTAGGGT1650.076.900313
GGAGCTA16850.076.697479
AGGGAAT11300.076.522245
Produced by FastQC (version 0.11.5)