FastQCFastQC Report
Mon 27 Feb 2023
SRR3127206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295076
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395091.1990315246143033No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT67940.20618644304410583No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG46990.14260672591466783TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC46280.14045199564441002TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG44540.13517138906659512TruSeq Adapter, Index 15 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42970.13040670382109548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120100.085.582021
ACGGGTA2250.073.108824
ATAGGGC18150.071.984543
TAGGGCA16300.071.218634
AGTAGGG55200.069.814542
AGGGAAT38000.069.754655
ATAGCGG7450.069.424351
GAGGGAT38250.068.9311754
TAGCGGG18550.068.912192
ATAGGGA27850.068.681593
AGGGATG48550.068.536475
ATGAGGG51900.068.45822
ACGGGAT8450.068.4120564
AGTAAGG22900.068.16761
AACGAGG16100.067.170321
GAATAGG19800.066.966771
TAGGGAT25250.066.821674
TAAGGGA32750.066.29823
AAGAGGG110900.066.1095352
AGGGCAT25150.065.5913855
Produced by FastQC (version 0.11.5)