FastQCFastQC Report
Mon 27 Feb 2023
SRR3127205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624027
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283331.0797526092528773No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53700.2046472844982159No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG42950.16367971823460656TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG39280.14969358165903018TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC36640.13963270957196705TruSeq Adapter, Index 21 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33370.1271709475550366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87250.085.737981
TAGGGCA12300.073.165694
ATAGGGA20700.072.8381353
AGGGAAT29600.072.555195
AGGGATG37750.072.0784765
CGTAGGG6050.070.870452
ATAGGGC14550.070.5947953
AGTAGGG44050.070.488572
TAGGGAC12900.070.1278844
ATGCGGG11700.069.6689762
AGGGCAT20650.068.9551245
GAATAGG14850.068.88031
TATAGGG15800.067.991872
GAGGGAT29800.067.829754
TAGCGGG12350.067.528252
TAGGGAT19300.067.1358644
GGTAAGG16200.066.9228741
GTAAAGG20050.066.76761
AAGAGGG84750.066.4921952
ACGGGAT6950.066.43864
Produced by FastQC (version 0.11.5)