FastQCFastQC Report
Mon 27 Feb 2023
SRR3127199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919827
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287960.9862228138858911No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG52860.181038123149077No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC49340.16898261438092052No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47970.1642905555705869No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG44230.15148157750442065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88250.082.977871
TAGGGCA15450.075.043094
ATAGGGC16750.074.565033
CGTAGGG8500.073.19252
GAATAGG16000.072.791811
AGTAAGG17800.072.053391
GTAGGGA17250.069.944723
TAGAGGG50000.069.754672
ATAGGGA23700.069.602333
ATAGCGG4900.069.285441
GTAGGGC12800.068.855043
ACGGGAT6900.068.30544
AGAGGGC46100.068.089073
ATAAGGG40350.067.631122
AGTAGGG43300.067.595112
GGATAGG18300.067.250411
TAGTAGG11650.067.187381
GGTAAGG16200.066.945031
AAGAGGG98600.066.729842
ATAGAGG18400.066.6286541
Produced by FastQC (version 0.11.5)