FastQCFastQC Report
Mon 27 Feb 2023
SRR3127198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027725
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298240.9850300142846528No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51030.16854238743611127No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG51020.16850935933745634No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC47800.15787431157056867No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG41290.13637301934620877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89400.083.2351461
ATAGCGG6100.078.701451
GAATAGG16200.077.572651
ACGGGTA3500.075.194564
ATAGGGC17200.074.866683
TAGGGCA16950.074.0300454
GGTAAGG15700.073.747591
CGTAGGG7550.072.8676762
ACGGGAT7800.072.302454
AGTAAGG18250.072.21171
AGTACGG4050.072.05251
TAAGGGT12300.070.303853
AGTAGGG46900.069.178682
ATAGAGG19100.068.751661
GTAGGGA20100.068.5074843
AGGGCAT24900.068.324375
ATAGGGA24050.067.807983
AATAGGG41300.067.40122
AGAGGGC48550.067.3730853
TAGAGGG53900.067.347972
Produced by FastQC (version 0.11.5)