FastQCFastQC Report
Mon 27 Feb 2023
SRR3127197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125546
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT574141.3916703389078682No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC90130.21846805247111534No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC82010.19878580919955807No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG67980.16477818935966296No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG62980.15265858143382718No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55520.13457612640848024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT175550.085.6281
TAGGGCA20950.076.27454
CGTAGGG11900.073.061432
ATAGGGA37000.073.0347443
AGGGATG65850.072.014485
ATAGGGC25150.071.755743
CTACGAC17050.071.39375
AGGGCAT37300.071.1908345
TACGACC17300.070.361996
TAGAGGG66350.070.051712
AGTAGGG56600.069.331962
AGGGAAT50350.069.074335
ATAAGGG54900.068.996352
TAGGGTA13350.068.297524
GGTAAGG25100.067.999681
GAGGGAT56550.067.983764
GTAGGGA31800.067.390883
TAAGGGA37550.067.334213
AATAGGG60350.066.5034262
GTAGGGC20650.066.2271963
Produced by FastQC (version 0.11.5)