FastQCFastQC Report
Mon 27 Feb 2023
SRR3127194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207033
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332101.0355365847498295No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC76140.23741570479630236No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41440.12921600744364026No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG41290.1287482854089746No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG40270.12556777557324791No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC38090.11877021533610661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108100.081.941281
CGTAGGG8600.077.0486452
AGGGATG63100.075.74545
ATAGGGA30750.072.2904053
GTAGGGT12250.071.7414553
TAGGGCA18050.071.080444
ATAGGGC20900.070.607053
GTAGGGA27550.068.916693
GGTAAGG17550.068.849941
AAGGGAT59350.068.574374
TAAGGGA30300.068.555823
AGGGAAT41450.068.255335
AGTAGGG50050.068.167422
AGGGCAT30350.068.133185
TAGGGTA12200.067.7980964
GTAGGGC17000.067.730123
ACGGGAT11600.067.253484
TATAGGG24100.066.883872
ATAGCGG7100.066.88221
TAAGAGG23300.066.791251
Produced by FastQC (version 0.11.5)