FastQCFastQC Report
Mon 27 Feb 2023
SRR3127190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885507
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78720.8889822440703462No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28240.31891334568783763No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG25520.2881964795309354No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG24920.2814207002316187No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC21590.24381512512041126Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG4650.086.183493
CGTTTTT30850.085.612771
CGGGATA2150.083.057445
TCCGCTA4950.082.8933641
CGTAGGG2050.082.804882
ATAGGGC6750.082.42073
AGGGATG26650.082.3485
AAGGGCA15400.080.824174
CGAAGGG7450.080.381242
TAGGGCG2350.080.2509464
ATAGCGG1300.079.81511
CTACGAC5150.079.3862845
GTAGGGC5200.078.881293
ACGGGAT3650.078.7943344
GAGGGAT18900.077.082314
GCGGGAT4050.076.8328554
AGGGCAT9400.076.488655
AGGGCAC7900.076.14065
GCTACGA5350.075.78844
ACGACCA5100.075.557417
Produced by FastQC (version 0.11.5)