FastQCFastQC Report
Mon 27 Feb 2023
SRR3127186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016909
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132691.304836519295237No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG54890.5397729787031091RNA PCR Primer, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53980.5308242920457976No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG47690.46897018317273226RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC41480.40790277202778225RNA PCR Primer, Index 38 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18370.1806454658184754RNA PCR Primer, Index 38 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT18080.17779368655405745RNA PCR Primer, Index 38 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT18020.17720366325797096No Hit
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT15600.1534060569824832RNA PCR Primer, Index 38 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT13390.13167353224329809No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG13170.129510113490981RNA PCR Primer, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGA12190.1198730663215686No Hit
GGAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT11080.10895763534396884No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT10760.10581084443150764No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10690.10512248391940675No Hit
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT10540.10364742567919055RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40850.087.545221
TCCGCTA10550.086.5286941
GCTACGA10500.085.491084
CGTAGGG2300.083.7950062
CGCTACG10750.083.065723
CGAAGGG8450.082.8880162
CTACGAC10850.082.73335
AGGGATG27900.082.2038965
TACGACC11200.079.7282646
TGCGGGC1550.078.834873
GGATGGC19050.078.6995857
GTAGGGT3450.077.648393
ATTGCGG1100.077.01
AAGGGCA12400.076.9398044
GATGGCA17800.076.833348
GATGACC16100.076.772678
GCGGGAT4050.076.588864
AACGAGG2400.076.465271
CGACCAA11500.076.422378
ATAGCGG1050.076.185191
Produced by FastQC (version 0.11.5)